Biopython Error - The System Cannot Find The File Specified

I have encountered an error which I am not able to resolve. I am trying to perform the easiest set of commands that will perform a tBLASTn algorithm, looking for a sequence (sequen

Solution 1:

What are the paths of the files?

The path r"\Biopython\cucumber.fasta", for example, is an absolute path on the current drive (because it starts with a backslash and no drive letter), which I think in your case is r"C:\Biopython\cucumber.fasta". Is that correct?